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我们曾用吡喹酮早期治疗感染日本血吸虫的小鼠后,可以抑制虫卵肝肉芽肿的形成。为探讨其抑制机理,我们应用鼠肺肉芽肿模型进行了进一步研究。观察体外用吡喹酮处理的日本血吸虫卵,在鼠体内形成肺肉芽肿的动态变化。材料和方法一、吡喹酮处理虫卵昆明远交鼠30只,雌雄不拘,每只小鼠经腹部皮肤人工感染日本血吸虫尾蚴80～100条。56d 后取肝脏,常规分离虫卵。分离纯卵用500μg/ml 浓度吡喹酮药液按1%(w/v)配成悬液,置4℃
Early treatment of mice infected with Schistosoma japonicum with praziquantel prevented the formation of egg granulomatous granuloma. To investigate its inhibitory mechanism, we further studied the model of mouse lung granuloma. Observe the in vitro praziquantel treatment of Schistosoma japonicum eggs in mice to form the dynamic changes of pulmonary granuloma. Materials and methods A, praziquantel treatment of eggs Kunming distant mice 30, male or female, each mouse artificially infected with cercariae of Schistosoma japonicum 80 to 100 by abdominal skin. After 56d, the liver was taken and the eggs were separated routinely. Isolation of pure eggs with 500μg / ml concentration of praziquantel solution 1% (w / v) dubbed the suspension, set 4 ℃