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本文对用于光动力癌症疗法(PPT)的新光敏剂铝酞菁(AlSPC)与人癌细胞的作用进行了实验探讨。细胞荧光光谱的测定及荧光强度的定量表明,铝酞菁能被人癌细胞所吸收,且经40μg/mlAlSPC培育24小时后,细胞对AlSPC吸收的量级为3.47μg/10~7细胞。对人癌细胞的光敏杀伤显示,杀伤效果正比于光敏剂培育浓度与光辐照剂量。由脂质过氧化的荧光研究表明,经光敏反应后人癌细胞膜的脂质过氧化代谢产物丙二醛(MDA)明显增高,揭示在AlSPC光敏反应中有活性氧作用。色氨酸降解的荧光实验证实,在光敏反应中有单线态氧(~1O_2)的生成。
In this paper, we investigated the effect of a new photosensitizer Al-phthalocyanine (AlSPC) used in photodynamic cancer therapy (PPT) on human cancer cells. The determination of the fluorescence spectrum and the quantification of the fluorescence intensity showed that the aluminum phthalocyanine was absorbed by human cancer cells, and the absorption of AlSPC by the cells was 3.47μg / 10 ~ 7 after 24h incubation with 40μg / ml AlSPC. Phototoxicity on human cancer cells showed that the killing effect is proportional to the photosensitizer incubation concentration and light irradiation dose. Fluorescence studies by lipid peroxidation showed that the lipid peroxidation metabolite malondialdehyde (MDA) of human cancer cell membrane was significantly increased after the photosensitization reaction, revealing that there was reactive oxygen species in AlSPC photosensitive reaction. Tryptophan degradation of fluorescence experiments confirmed that in the photosensitive reaction singlet oxygen (~ 1O_2) generation.