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目的研究体外诱导脐m CD8’细胞毒性T淋巴细胞(CTL)特异性杀伤门m病细胞的作用,探讨脐m淋巴细胞用于特异性免疫治疗的可行性i方法联合细胞因子体外诱导】O份脐m单个核细胞(MNC)分化为树突细胞(DC),同时负载U937冻融抗原;成熟DC刺激同源的脐m T淋巴细胞成为CTL,经MACS磁珠分选出CD8’CTL倒置显做镜、扫描电予硅微镜及流式细胞术等厅法检测DC细胞特性..MTT法测定细胞杀伤活性。结果 10份脐fli【标本均可培养出形态典型、功能成熟的DC?CD8’CTL、C1)8一CTL和淋巴细胞(TL)组对U937细胞不蚓效靶比的杀伤率以CD8’CTI.组景高;CD8’CTl。存40:l效靶比时对靶细胞U937细胞的杀伤率高于对K562细胞的杀伤率[分别为(66.36±12.43)％和(41.97±14.24)％,(P<0.05)1.而CD8 CTL在40:l效靶比时对U937细胞干“K562细胞的杀伤率差异无统汁学意义f分别为(34.47±8.19)％和(22.45±4.()O)％(尸>O.05)]结论用负载U937细胞抗原的成熟脐InL DC,诱导dj脐山l_淋巴细胞特异性的CD8’CTL;CD8’CTL对U937细胞的杀伤活性强于CD8一CTI.;CD8’CTI,对U937细胞的杀伤活性具有特异性。
OBJECTIVE: To study the role of umbilical mCD8 ’cytotoxic T lymphocytes (CTLs) in killing m cell in vitro and to explore the feasibility of umbilical m-lymphocytes for specific immunotherapy in vitro. Umbilical m mononuclear cells (MNCs) differentiated into dendritic cells (DCs), while loading U937 freeze-thaw antigen; mature DC stimulated homologous umbilical m T lymphocytes become CTL, MACS beads were sorted out CD8’CTL inverted To do mirror, scanning electron micrographs and flow cytometry and other hall tests DC cell characteristics .. MTT assay of cell killing activity. Results 10 cases of umbilical fli 【】 specimens can be cultured morphologically typical, mature DC? CD8’CTL, C1) 8-CTL and lymphocyte (TL) group on U937 cells leprosy effect target ratio to CD8’CTI Group King; CD8’CTl. The killing rate of target cell U937 cells was higher than that of K562 cells ([(66.36 ± 12.43)% vs (41.97 ± 14.24)%, P <0.05] There was no significant difference in killing rates of CTLs between K562 cells and U937 cells when CTL was 40: 1 (f = 34.47 ± 8.19% and (22.45 ± 4.40)%, respectively. 05)] Conclusion Mucous U937 cell antigen loaded with mature umbilical InL DC induced dj umbilical cord l_ lymphocyte-specific CD8’CTL; CD8’CTL on U937 cells cytotoxic activity stronger than CD8 a CTI; CD8’CTI, The killing activity of U937 cells is specific.