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文献报导先天性巨结肠症为一多因素遗传性疾病,且有地区种族的差别。长期以来,人们对其病理生理、诊断方法及治疗方法进行了大量的研究,但是对患儿血清及病变狭窄段肠管及近端正常肠段的肠壁内某些微量元素含量,尚未见有述及。为了探讨微量元素与巨结肠症之间关系,作者进行了这方面的测试,以期了解彼此间有无差异。材料与方法一、1986年9月～1987年9月共收集巨结肠手术病儿血清及肠壁标本。年龄最小11月,最大5岁。男18例,女4例。对照组10例系住院非消化道疾病患儿(如疝、尿道下裂、鞘膜积液)。二、标本收集:按去离子要求,采集术前空腹静脉血,经双离心除尽红细胞后抽吸其血清存于去离子试管内待测。手术时切取狭窄段及近心端正常肠管全层各1×1cm~2,用生理盐水及双蒸水冲洗干净,置烤箱内烤干后放于密封干燥器内。三、血清经处理后以日立180—80型原子吸收光谱仪测定。
Hirschsprung’s disease reported in the literature as a multi-factor genetic disease, and regional differences in race. For a long time, a great deal of researches have been done on its pathophysiology, diagnosis and treatment methods. However, there is not yet any trace element content in the intestine wall of intestinal tract and normal intestinal segment of children with stenosis and stenosis and. In order to explore the relationship between trace elements and megacolon disease, the authors conducted tests in this area to see if there was any difference between them. Materials and methods First, from September 1986 to September 1987 collected a total of patients with giant colon surgery serum and intestinal wall specimens. The youngest in November, up to 5 years old. 18 males and 4 females. The control group of 10 patients were hospitalized with non-gastrointestinal diseases (such as hernia, hypospadias, hydrocele). Second, the collection of specimens: According to deionization requirements, preoperative fasting venous blood collection, after double centrifugation, remove the red blood cells, the serum was stored in deionized test tube to be tested. Surgical removal of the narrow segment and the proximal end of the normal intestinal full-thickness 1 × 1cm ~ 2, rinse with saline and double distilled water, oven placed in the oven dried and placed in a sealed dryer. Third, the serum treated with Hitachi 180-80 atomic absorption spectrometry.