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目的 研究辛伐他丁对胃癌细胞增殖的影响。 方法 采用甲唑蓝 (MTT)比色法和流式细胞仪检测胃癌细胞的增殖活性和细胞周期。 结果 辛伐他丁可明显降低MNK4 50 胃癌细胞增殖活性、S期细胞比和PI值 ,在 >1μM浓度时 ,与对照组比较具有显著性差异 (P <0 .0 5 ) ,同时加入甲羟戊酸(MVA)则可逆转辛伐他丁的抑制作用。另外 ,辛伐他丁对胃癌细胞经过G2 期也具有一定阻抑作用。 结论 辛伐他丁可抑制胃癌细胞生长增殖 ,提示辛伐他丁具有抗肿瘤作用 ,可作为胃癌辅助治疗的新手段。
Objective To study the effect of simvastatin on proliferation of gastric cancer cells. Methods MTT colorimetric assay and flow cytometry were used to detect the proliferation activity and cell cycle of gastric cancer cells. Results Simvastatin significantly decreased the proliferative activity of MNK4 50 gastric cancer cells, the cell ratio in S phase, and the PI value. At a concentration of >1 μM, there was a significant difference compared with the control group (P < 0.05). Valproic acid (MVA) reverses the inhibitory effect of simvastatin. In addition, simvastatin has a certain inhibitory effect on gastric cancer cells after G2. Conclusion Simvastatin can inhibit the growth and proliferation of gastric cancer cells, suggesting that simvastatin has anti-tumor effect and can be used as a new method for adjuvant treatment of gastric cancer.