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[Objectives] To determine the content of total flavonoids in Tetracera asiatica from different production areas in Guangxi and provide a basis for improving the quality standards of crude drug. [Methods] Using 50% ethanol as extractor,NaNO_2-Al(NO_3)_3-NaOH as chromogenic system and rutin as the reference substance,the content of total flavonoids from the crude drug was determined. [Results] A method for determining the content of total flavonoids was established,the content of total flavonoids was 11.35-21.35 mg·g~(-1),and the average content was 17.64 mg·g~(-1). [Conclusions] The method had high repeatability and stability,and the method was simple,rapid and sensitive and suitable for quantitative analysis.
[Objectives] To determine the content of total flavonoids in Tetracera asiatica from different production areas in Guangxi and provide a basis for improving the quality standards of crude drug. [Methods] Using 50% ethanol as extractor, NaNO_2-Al (NO_3) _3- NaOH as chromogenic system and rutin as the reference substance, the content of total flavonoids from the crude drug was determined. [Results] A method for determining the content of total flavonoids was established, the content of total flavonoids was 11.35-21.35 mg · g ~ (-1), and the average content was 17.64 mg · g -1 [Conclusions] The method had high repeatability and stability, and the method was simple, rapid and sensitive and suitable for quantitative analysis.