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目的 探讨血管成行术后再狭窄的预防。方法 以兔右颈总动脉内膜剥脱为实验模型，３０只兔随机分为单纯损伤组（ｎ＝ １２）、损伤＋ 药物治疗组（简称药物治疗组，ｎ＝ １２）和假手术组（ｎ＝ ６），术后１４ 天行病理形态学观察及增殖细胞核抗原（Ｐｒｏｌｉｆｅｒａｔｉｎｇ ｃｅｌｌｎｕｃｌｅａｒ ａｎｔｉｇｅｎ，ＰＣＮＡ）免疫组化分析。结果 药物治疗组右颈总动脉内膜平均厚度显著小于单纯损伤组（３５．８１±１６．１３ μｍ ｖｓ ７２．１４±１４．６６μｍ ，Ｐ＜ ０．０１）；药物治疗组右颈总动脉狭窄度显著小于单纯损伤组（２１．７４％ ±８．９９％ ｖｓ ５３．６８％ ±１５．５７％ ，Ｐ＜ ０．０１）；药物治疗组细胞增殖指数显著小于单纯损伤组（０．２３０±０．０４７ ｖｓ ０．４２９±０．０５５，Ｐ＜ ０．０１）。结论 蛋白激酶Ｃ（ｐｒｏｔｅｉｎ ｋｉｎａｓｅ Ｃ，ＰＫＣ）特异抑制剂多粘菌素Ｂ联用Ｃａ２＋ 通道阻滞剂维拉帕米能有效抑制受损血管内膜增生，对预防再狭窄可能具有潜在临床应用前景
Objective To investigate the prevention of restenosis after angioplasty. Methods Thirty rabbits were randomly divided into simple injury group (n = 12), injury + drug treatment group (n = 12) and sham operation group (n = 12) = 6) .Pathological examination and Proliferating cell nuclear antigen (PCNA) immunohistochemistry were performed 14 days after operation. Results The average thickness of the right common carotid artery was significantly lower in the drug-treated group than that in the simple injury group (35.81 ± 16.13 μm vs 72.14 ± 14.66μm, P <0.01). In the drug-treated group, the right common carotid artery stenosis (21.74% ± 8.99% vs 53.68% ± 15.57%, P <0.01). The proliferation index of the drug-treated group was significantly lower than that of the simple injury group (0.230 ± 0.047 vs 0.429 ± 0.055, P <0.01). Conclusion The specific inhibitor of protein kinase C (PKC) polymyxin B combined with verapamil, a Ca2 + channel blocker, can effectively inhibit the intimal hyperplasia of impaired vessels and may have potential clinical application in the prevention of restenosis