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探讨IFN-γ与IL-12分别对细胞因子诱导的杀伤细胞(cytokine-induced killer,CIK)的体外扩增速度、细胞免疫表型和杀伤活性的影响。分别以传统的IFN-γ、CD3 mAb、IL-2和IL-1α以及IL-12、CD3 mAb、IL-2和IL-1α为诱导剂制备CIK细胞,直接活细胞计数法比较两者的扩增速度,流式细胞仪检测比较两组细胞的免疫表型,LDH法比较两者对K562细胞株的杀伤活性。结果:两种CIK细胞的增殖速度无显著性差异,但IL-12启动的CIK细胞体外扩增时存活时间比常规CIK细胞长,并且其CD3+CD8+及CD3+CD56+细胞显著高于常规CIK细胞(P<0.05),CD3+CD4+和CD4/CD8显著低于常规CIK(P<0.05),并且其体外对K562的非特异杀伤活性在效靶比为40∶1时显著高于常规CIK。IL-12诱导扩增的CIK中效应细胞比例显著增加,同时抑制性T细胞比例降低,且体外该CIK细胞的杀伤能力较常规CIK好。故IL-12取代IFN-γ对CIK进行预刺激可增强CIK细胞的生物学活性。
To investigate the effects of IFN-γand IL-12 on the proliferation, cytotoxicity and cytotoxicity of cytokine-induced killer (CIK) in vitro. CIK cells were prepared by using traditional IFN-γ, CD3 mAb, IL-2 and IL-1α as well as IL-12, CD3 mAb, IL-2 and IL- Growth rate and flow cytometry were used to compare the immunophenotypes of the two groups of cells. LDH assay was used to compare the killing activity of K562 and K562 cells. Results: There was no significant difference in the proliferation rate between the two CIK cells. However, CIK cells activated by IL-12 had longer survival time than CIK cells in vitro and their CD3 + CD8 + and CD3 + CD56 + cells were significantly higher than that of CIK cells (P <0.05). CD3 + CD4 + and CD4 / CD8 were significantly lower than that of CIK (P <0.05), and the non-specific killing activity of K562 in vitro was significantly higher than that of conventional CIK when the target ratio was 40:1. The proportion of effector cells in CIK induced by IL-12 was significantly increased, while the proportion of suppressor T cells was decreased. In addition, the cytotoxicity of CIK cells in vitro was better than that of CIK. Therefore, IL-12 in place of IFN-γ CIK pre-stimulation can enhance CIK cell biological activity.