Molecular differentiation of cryptic stage ofEchinococcus granulosusand Taenia species from faecal a

• 作 者: Diganta Pan Sumanta De Asit Kumar Bera Subhashis Bandyopadhyay Subrata Kumar Das Debasis Bhattacharya
• 机 构: Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata-700 037,
• 出 处: 亚太热带医药杂志（英文版）
• 发表日期: 2010年4期
• 关键词: Taenia solium Echinococcus granulosus Environmental samples PCR- RFLP

Objective:To differentiate cryptic stage ofEchinococcus granulosus(E. granulosus) andTaenia by PCR-RFLP and sequence information of amplicon.Methods: DNA were isolated from metacestodes stage ofTaenia andE. granulosus using DNA isolation kit (Q-BIOgene kit, USA), the amplified and purified DNA product was then cloned and sent for sequencing. The generating sequence information was used for amplicons identification.Results:Out of 112 faecal and environmental samples, 16 exhibited positive result. The product size of amplicon positive for E. granulosus was 310 bp; whereas, forTaenia spp. sizes varied from 379 to 388 bp. Restriction profile of actin II with Csp61 also differedTaenia spp. andE. granulosus.Conclusions: The result of the study indicated that, the primers were useful to differentiate cryptic stage of the two genera which is yet to be reported earlier.