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肝硬化患者常有血液凝固异常,但有关其血小板功能异常的报告甚少。本文研究酒精性肝硬化和原发性胆汁性肝硬化病人的血小板凝集和ATP释放异常。患者和方法:随机选择经病理确诊的酒精性肝硬化和原发性胆汁性肝硬化各10例,正常对照组10例。取静脉血抗凝离心,作血小板计数。用比浊法测定血小板凝集,再加促凝物质骨胶或ADP后测血小板凝集,算出前后两次血小板凝集的最大透光度百分率(T_(max)%)。测定促凝时间。以萤光素酶法测定血小板释放入血浆之ATP量,与已知ATP标准量比较。最后分组进行统计比较。结果:三组在加骨胶和ADP后血小板凝集的T_(max)%分别为:酒精性肝硬化组48.4±14.9和32.8
Cirrhotic patients often have abnormal blood coagulation, but little is known about their abnormal platelet function. This article investigates abnormal platelet aggregation and ATP release in patients with alcoholic cirrhosis and primary biliary cirrhosis. PATIENTS AND METHODS: Ten patients with pathologically confirmed alcoholic cirrhosis and primary biliary cirrhosis were randomly selected, and 10 patients in the normal control group. Take venous blood anticoagulation for platelet count. The platelet aggregation was measured by turbidimetry. The platelet aggregation was measured by procoagulant glue or ADP. The percentage of maximum transmittance (T max%) of platelet aggregation before and after treatment was calculated. Determination of procoagulant time. Luciferase assay was used to determine the amount of ATP released from platelets into the plasma, compared to the known ATP standard. The last group for statistical comparison. Results: The T_ (max)% of platelet aggregation in three groups after adding glue and ADP were: 48.4 ± 14.9 and 32.8 in alcoholic cirrhosis group