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本文应用免疫细胞化学(整装分层铺片和恒冷箱冰冻切片)和放射免疫测定技术,对大鼠小肠降钙素基因相关肽(CGRP)的分布特征及其在肠型放射病时的变化进行了观察。 结果表明:小肠各层均有丰富的CGRP-免疫反应性(CGRP-Ⅰ)神经,以肌间神经丛、粘膜下神经丛和粘膜神经丛密度为高,小肠壁内小血管及肠腺周围也较丰富,在肌间和粘膜下神经丛中见有一些CGRP-Ⅰ神经细胞。肠型放射病时,小肠CGPR水平有一双相改变,24h下降,48和72h升高。 以上结果提示,CGRP可能与胃肠道运动,吸收,分泌,感觉和局部血流的调节有关,CGRP在AIRS时可能有一应激性释放,并通过多种途径,尤其是影响局部血流和增加血管的通透性,参与AIRS的损伤机制。
In this study, the distribution characteristics of calcitonin gene-related peptide (CGRP) in rat intestine and its changes in intestinal type radiation sickness were studied by immunocytochemistry (stratified layered dressing and cryostat) and radioimmunoassay Observed. The results showed that all the layers of the small intestine were rich in CGRP-I nerves. The density of myenteric plexus, submucosal plexus and mucosal nerve plexus was high. The small blood vessels in the small intestine and the surrounding gut were also Richer, in the intermuscular and submucosal plexus see some CGRP-I nerve cells. Intestinal radiation sickness, small intestine CGPR level of a biphasic change, 24h decreased, 48 and 72h increased. The above results suggest that CGRP may be related to the regulation of gastrointestinal motility, absorption, secretion, sensory and local blood flow. CGRP may have a stress release in AIRS and may be affected by a variety of pathways, especially affecting local blood flow and increase Vascular permeability, involved in AIRS injury mechanism.