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目的：了解ＨＣＶ不同基因型对α干扰素治疗的反应。方法：采取干扰素不同治疗量和不同疗程治疗病人。血清用逆转录酶一聚台酶链反应扩增ＨＣＶＲＮＡ５－非编码区，再用Ｓｌｏｔ－ｂｌｏｔ杂交鉴定。结果：ＨＣＶＲＮＡ阴转，丙氨酸转氨酶（ＡＬＴ）降至正常（完全反应）占４０．０％；３ｘ１０￣６Ｕ治疗一月无反应，增量６ｘ１０￣６Ｕ出现前述结果（部分反应Ｉ）占３６．７％：增量６ｘ１０￣６Ｕ治疗一月无反应，再增量９ｘ１０￣６Ｕ出现前述完全反应结果（部分反应Ⅱ）占１５．０％；增量９×１０￣６Ｕ治疗一月无反应（无反应）占８．３％。３８．６％ＨＣＶ基因１型、４４．４％Ⅱ型、４２．９％Ⅲ型是完全反应。２４例完全反应病人中１５例治疗前ＨＣＶＲＮＡ拷贝数低于１０／ｍｌ。结论：各基因型对于扰索的治疗反应无显著性差异，肝损害轻、血清ＨＣＶＲＮＡ拷贝数低者对干扰素治疗反应较好。
Objective: To understand the different genotypes of HCV response to interferon alpha. Methods: Interferon treatment with different doses and different treatment of patients. HCV RNA5-non-coding region was amplified by reverse transcriptase-polymerase chain reaction and identified by Slot-blot hybridization. Results: HCVRNA was negative and ALT decreased to normal (complete response) 40.0%; 3x10 ~ 6U no response in January, 6x10 ~ 6U increments The above results (partial reaction I) accounted for 36 .7%: an increase of 6x10 ~ 6U no response to treatment in January, and then increase the amount of 9x10 ~ 6U complete response results (partial response Ⅱ) accounted for 15.0%; an increase of 9 × 10 ~ 6U no response to January treatment ( No reaction) accounted for 8.3%. 38.6% of HCV genotype 1, 44.4% of type Ⅱ, 42.9% of type Ⅲ were completely reactive. Fifteen of 24 patients with complete response had HCVRNA copy number less than 10 / ml before treatment. CONCLUSION: There is no significant difference in the response of each genotype to the treatment of interferon. Light hepatic damage and low serum HCVRNA copy number respond well to interferon treatment.