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目的 探讨脂多糖 (LPS)对枯否细胞 (KC)CD14和ToLL样受体 4(TLR4)表达的影响。方法 应用不同浓度 (10mg/L～ 1μg/L)或同一浓度 (10mg/L)的LPS在不同时间 (0 .5 2 4.0h)刺激培养大鼠 48h的KC并测定其CD14和TLR4、肿瘤坏死因子 (TNF)和白细胞介素 6(IL 6)表达的变化。结果 LPS能明显上调KC中CD14、TLR4表达 ,且其表达量与LPS浓度 (10mg/L ,CD14 /GAPDH =0 .73 ,TLR4/GAPDH =0 .63 )及时间呈正相关 ,其中CD14的表达在 3～ 6h左右达到高峰CD14 /GAPDH =0 .86) ;同时 ,KC产生的活性介质能明显上调其CD14、TLR4的表达(CD14 /GAPDH =0 .70 ,TLR4/GAPDH =0 .5 9)。结论 LPS及其刺激KC后产生的活性介质与CD14、TLR4表达密切相关 ,在 1 3h的CD14、TLR4表达的增强可能主要由LPS引起 ,而此后的进一步增强可能与KC释放的细胞因子密切相关。
Objective To investigate the effect of lipopolysaccharide (LPS) on the expressions of CD14 and TLR4 in Kupffer cells. Methods KCs cultured at different concentrations (10mg / L ~ 1μg / L) or LPS at the same concentration (10mg / L) were cultured at different time (0.52 4.0 hours) to determine the CD14 and TLR4, tumor necrosis Changes in the expression of factor (TNF) and interleukin 6 (IL 6). Results LPS significantly up-regulated the expression of CD14 and TLR4 in KC, and the expression of CD14 was positively correlated with the concentration of LPS (10mg / L, CD14 / GAPDH = 0.73, TLR4 / GAPDH = 0.63) CD14 / GAPDH = 0.86). At the same time, the active mediators produced by KC significantly up-regulated the expression of CD14 and TLR4 (CD14 / GAPDH = 0.70, TLR4 / GAPDH = 0.59). Conclusions The expressions of CD14 and TLR4 in LPS and its active mediators are closely related to the expression of CD14 and TLR4. The enhanced expression of CD14 and TLR4 at 13 h may be mainly caused by LPS, but the further enhancement may be related to the release of cytokines by KC.