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目的 探讨美蓝在家兔失血性休克中的作用。方法 复制不可逆失血性休克家兔模型 ,2 8只雄性家兔随机分为美蓝组 (MB ,n =15 )和生理盐水组 (NS ,n =13) ,监测两组休克前后SBP、DBP、MAP的动态变化及血清NO的水平。结果 (1)MB组与NS组相比 ,静注开始 10min之后SBP、DBP、MAP显著升高 (P <0 0 1) ,其升高效应维持约 4 0min。 (2 )失血性休克后 ,两组的血清NO水平均有一定程度上升。两组间相比 ,仅休克 4 5′点 ,MB组较NS组显著降低 (P <0 0 5 )。 (3)MB组存活时间 (10 9 6 7± 2 2 2 4 )min显著长于NS组 (6 7 85± 17 77)min ,90min存活率显著高于NS组。结论 在家兔不可逆失血性休克时早期应用 7 5mg/kg的美蓝 ,能快速升高血压 ,延长动物的存活时间 ,并能一过性降低血清NO水平。推测美蓝的升压效应可能有部分是通过降低NO水平而起作用。
Objective To investigate the role of methylene blue in hemorrhagic shock in rabbits. Methods Rabbit models of irreversible hemorrhagic shock were duplicated. Twenty eight male rabbits were randomly divided into methylene blue group (MB, n = 15) and normal saline group (NS, n = 13) Dynamic changes of MAP and serum NO levels. Results (1) SBP, DBP and MAP significantly increased (P <0.01) in MB group compared with NS group at 10 minutes after the start of intravenous injection, and its up-regulation effect was maintained at about 40 minutes. (2) After hemorrhagic shock, serum NO levels in both groups increased to some extent. Compared with the NS group, the number of shocked rats was only 45% in the MB group (P <0.05). (3) The survival time of MB group was significantly longer than that of NS group (109 7 ± 2 2 4 4 min 6 7 85 ± 17 77 min), and the survival rate of 90 min was significantly higher than that of NS group. Conclusion Early application of 75mg / kg methylene blue in rabbits with irreversible hemorrhagic shock can rapidly increase blood pressure, prolong the survival time of animals and reduce the level of serum NO transiently. It is speculated that the boosting effect of methylene blue may partly work by lowering the NO level.