Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique

• 作 者: 张洪艳 陆珩 李巍 梁汝强 金奎娟 周岳亮 阮康成 杨国桢
• 机 构: Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese
• 出 处: Chinese Physics B
• 发表日期: 2008年06期
• 关键词: labelling reflectivity oblique incidence format biochemical spots illumination a

This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI-RD) analysis without any labelling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.

This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI-RD) analysis without any labeling agents. The OI-RD intensities not only depend on the protein structure, but also vary with the The concentration of The method that has the potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.