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本文采用水热法合成N-乙酰-L-半胱氨酸稳定的碲化镉量子点(CdTe quantum dots,CdTe QDs),采用配体置换的方法制备得到氨基脱氧葡萄糖(2-amino-2-deoxy-D-glucose,DG)、聚乙二醇(polyethylene glycol,PEG)与9聚精氨酸(9-D-arginine,9R)共同修饰的碲化镉量子点(9R/DG-CdTe QDs)。通过紫外、荧光光谱、傅里叶红外光谱、核磁共振氢谱、高效液相-质谱联用、聚丙烯酰胺凝胶电泳和透射电子显微镜等手段对该复合量子点进行表征,并对该复合纳米粒的生物相容性、肿瘤靶向性及穿细胞膜的效果进行考察。结果表明,通过配体置换的方法可以成功构建DG、9R、PEG修饰的CdTe QDs复合纳米粒。TEM结果显示,该纳米粒分散性较好,粒径约为8~10 nm。化学修饰后的CdTe QDs吸收峰从480 nm红移至510 nm,发射峰从627 nm红移至659 nm。通过DG、9R、PEG的修饰还能进一步改善量子点的生物相容性,提高对葡萄糖转运体1高表达的肿瘤细胞的靶向性,增加量子点穿过细胞膜进入细胞浆的作用。
In this paper, CdTe quantum dots (CdTe QDs) stabilized by N-acetyl-L-cysteine were synthesized by hydrothermal method and 2-amino-2- (9R / DG-CdTe QDs) modified with polyethylene glycol (PEG) and 9-D-arginine (9R) . The composite quantum dots were characterized by ultraviolet, fluorescence spectroscopy, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, high performance liquid chromatography-mass spectrometry, polyacrylamide gel electrophoresis and transmission electron microscopy, and the composite nano Particle biocompatibility, tumor targeting and the effect of wearing cell membranes were investigated. The results showed that DG, 9R, PEG modified CdTe QDs composite nanoparticles could be successfully constructed by ligand exchange method. TEM results showed that the nanoparticles were well dispersed and the particle size was about 8 ~ 10 nm. The chemical modification of the CdTe QDs red shift from 480 nm to 510 nm, the emission peak red-shifted from 627 nm to 659 nm. The modification of DG, 9R and PEG can further improve the biocompatibility of QDs, enhance the targeting of tumor cells highly expressed by glucose transporter 1, and increase the effect of quantum dots passing through the cell membrane into the cytoplasm.