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为了阐明乙醇中毒对神经系统损害的机理 ,用0 .5- 5.0 g· L-1浓度的乙醇处理原代培养的 CD新生大鼠大脑皮层星形细胞 ,用反转录 PCR和Northern印迹杂交方法同时测定神经营养因子( NGF)的基因表达水平 .结果显示 0 .5g·L-1乙醇在染毒 2 4 h后 ,NGF m RNA水平开始降低 ,5.0 g· L-1在染毒 48或 72 h后 ,NGF m RNA水平与对照组比较降低了 50 % - 58% .结果表明 ,乙醇中毒在体外可降低新生大鼠星形细胞 NGF m RNA的生成 ,这种作用可干扰神经元与胶质细胞间的正常交互作用并可能与胚胎发育期乙醇接触导致的神经病理改变有关 .
To clarify the mechanism of ethanol poisoning on the nervous system, primary cultured neonatal rat cortical astrocytes were treated with ethanol at a concentration of 0.5-5.0 g · L-1. Reverse transcription PCR and Northern blot hybridization At the same time, the gene expression level of neurotrophic factor (NGF) was measured.The results showed that the level of NGF m RNA began to decline when 0.5 g · L-1 ethanol was applied for 24 h, while the concentration of 5.0 g · L- h, the level of NGF m RNA decreased by 50% -58% compared with the control group.The results showed that ethanol poisoning could reduce the production of NGF m RNA in astrocytes in neonatal rats in vitro, which could interfere with neuronal and glial The normal interaction between cells and may be related to the neuropathological changes caused by ethanol exposure during embryonic development.