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为明确NDPK1在细胞分裂过程中的作用及作用机制, 本研究利用过表达技术, 分析过表达NDPK1转基因拟南芥的株高和茎粗;用扫描显微镜观察比较过表达NDPK1转基因拟南芥细胞大小和形态、统计单位面积中细胞数目;利用RT-PCR技术, 检测过表达NDPK1转基因拟南芥中细胞分裂相关基因的表达情况, 初步分析NDPK1对细胞周期的调控机理.结果表明:过表达NDPK1转基因拟南芥下胚轴长度高于Col-0野生型拟南芥;过表达NDPK1转基因拟南芥的下胚轴细胞数目多于Col-0野生型拟南芥;过表达NDPK1中细胞分裂的促进因子CDC2和CycD2的表达量明显高于Col-0野生型拟南芥.以上结果表明, NDPK1可通过调控CDC2和CycD2的表达进而促进细胞分裂.“,”To clear the function and the action mechanism of NDPK1 in cell division, we analyzed the plant height and stem diameter of the NDPK1 over-expressing transgenic Arabidopsis using over-expression techniques in this study. The size and morphology of the NDPK1 over-expressing transgenic Arabidopsis cells were observed by scanning microscope and the number of cells per unit area was counted. RT-PCR technology was used to test the expression level of the cell division related genes in the NDPK1 over-expressing transgenic Arabidopsis, and preliminarily analyze the regulation mechanism of NDPK1 in cell cycle. The results showed that the length of hypocotyl in NDPK1 over-expressing transgenic Arabidopsis was longer than Col-0 wild-type Arabidopsis thaliana. The number of hypocotyl cell in NDPK1 over-expressing transgenic Arabidopsis was larger than Col-0 wild-type Arabidopsis thaliana. The expression level of CycD2, CDC2 which could shorten cell division cycle in NDPK1 over-expressing transgenic Arabidopsis was higher than that in Col-0 wild-type Arabidopsis thaliana. In conclusion, NDPK1 could regulate the expression of CDC2 and CycD2 genes to promote the progress of cell division.