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目的 建立实用的小密低密度脂蛋白(sdLDL)测定方法， 观察冠心病患者血浆中sdLDL和脂类成分的变化及相互关系。方法 采用2%～16%非变性聚丙烯酰胺梯度胶电泳和凝胶成象分析系统， 测定了105名冠心病患者和90名健康对照者血浆sdLDL、 血脂。结果 sdLDL＞50%的检出率， 冠心病组为38.1%， 对照组为11.1%(P＜0.01)；sdLDL增多患者的甘油三酯水平(2.43±1.22) mmol/L明显高于sdLDL正常者(1.46±0.64) mmol/L， P＜0.01。结论 sdLDL与冠心病发生发展有关， 且与甘油三酯关系密切；sdLDL测定可作为预测冠心病危险性的指标之一。
Objective To establish a practical method for determination of sdLDL and to observe the changes and correlations of plasma sdLDL and lipid components in patients with coronary heart disease. Methods Serum sdLDL and lipids were measured in 105 patients with coronary heart disease and 90 healthy controls using 2% ~ 16% non-denaturing polyacrylamide gradient gel electrophoresis and gel imaging system. Results The detection rate of sdLDL> 50% was 38.1% in coronary heart disease group and 11.1% in control group (P <0.01). The triglyceride level in patients with increased sdLDL (2.43 ± 1.22) mmol / L was significantly higher than that in sdLDL Normal (1.46 ± 0.64) mmol / L, P <0.01. Conclusion sdLDL is associated with the occurrence and development of coronary heart disease, and is closely related to triglyceride. SdLDL assay can be used as one of the indicators to predict the risk of coronary heart disease.