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为探讨不同疗程亚低温对新生儿脑损伤的保护作用，建立新生儿缺血缺氧性脑损伤亚低温干预大鼠模型。先在７日龄清洁级ＳＤ大鼠上以右颈总动脉结扎术和吸入低氧浓度气体法制备缺血缺氧性脑损伤（ＨＩＢＤ）模型，再将其随机分为室温恢复组（ＲｏＴ组，ｎ＝４４）和３种疗程（２４ｈ，４８ｈ，７２ｈ）的亚低温干预组（Ｈｙ２４组，ｎ＝２６；Ｈｙ４８组，ｎ＝１４；Ｈｙ７２组，ｎ＝２０）。亚低温干预期间采用配方奶喂养，并监测脑温、血糖、血气和血浆乳酸水平。实验２周后以体重增加百分率（ＷＩＰ）、脑组织病变情况（右侧脑损伤分数和右侧海马细胞死亡率）判定疗效。结果，配方奶喂养的亚低温干预组的血糖维持在正常范围内（＞３ｍｍｏｌ／Ｌ），脑温降低４～５℃；ＨＩＢＤ后各组均出现酸中毒，ＨＩＢＤ后６ｈＲｏＴ组的血气和血乳酸水平仍明显增高，而亚低温组已恢复正常。实验２周后Ｈｙ２４组的ＷＩＰ明显高于ＲｏＴ组（分别为１３４．５％、８８．０％，Ｐ＜０．０１），Ｈｙ４８与ＲｏＴ组之间无明显差异（Ｐ＞０．０５）。病理学检查结果显示２４～７２ｈ的亚低温干预使脑损伤程度降低了５３％～８９％，Ｈｙ７２组疗效最佳。这表明新生儿ＨＩＢＤ亚低温干预大鼠模型已成功建立，ＨＩＢＤ后?
To investigate the protective effects of hypothermia with different courses of treatment on neonatal brain injury, a rat model of hypothermia after neonatal hypoxic-ischemic brain damage was established. Hypoxic-ischemic brain damage (HIBD) models were established on the 7-day-old clean SD rats by right common carotid artery ligation and inhalation of hypoxia gas, and then were randomly divided into the room temperature recovery group (RoT group) , N = 44) and mild hypothermia intervention group (Hy24 group, n = 26; Hy48 group, n = 14; Hy72 group, n = 20) for 3 courses of treatment (24h, 48h, 72h). Mild hypothermia intervention period using formula feeding, and monitoring of brain temperature, blood glucose, blood gas and plasma lactate levels. Two weeks after the experiment, the effect of WIP and brain lesions (right brain injury score and right hippocampal cell death rate) was evaluated. As a result, the blood glucose of the mild hypothermia intervention group was maintained within the normal range (> 3mmol / L) and the brain temperature was lowered by 4 ~ 5 ℃. Acidosis was observed in all groups after HIBD. Blood gas and blood lactate The level is still significantly higher, while the mild hypothermia group has returned to normal. The WIP in Hy24 group was significantly higher than that in RoT group (134.5%, 88.0%, P <0.01, respectively) after 2 weeks of experiment. There was no significant difference between Hy48 and RoT group (P> 0.05). The results of pathological examination showed that mild hypothermia intervention at 24-72h decreased brain injury by 53% -89%, and Hy72 group had the best effect. This shows that neonatal HIBD mild hypothermia rat model has been successfully established, HIBD?