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目的：建立大鼠脑脓肿模型，探讨脓肿周围组织ＡＴＰ酶活性改变与脑水肿的关系．方法：大鼠右额叶定向注入细菌悬液（１μｌ，含金黄色葡萄球菌１０４个），接种后１～１９ｄ测定脓肿周围脑组织ＡＴＰ酶活性及水含量．结果：大鼠接种细菌后均在右额叶内形成脓肿，其病理过程与临床情况相似；脓肿周围组织ＡＴＰ酶活性在早期（７ｄ内）持续性降低，而水含量则进行性升高．脑水肿与组织Ｎａ＋Ｋ＋－ＡＴＰ酶（ｒ＝－０．８６５），Ｃａ２＋ＡＴＰ酶（ｒ＝－０．８８１）活性改变之间呈高度负相关（Ｐ＜０．０１）．结论：大鼠脑内特定部位定量种植细菌可稳定复制脑脓肿模型，脓肿周围组织ＡＴＰ酶活性抑制与脑水肿发生发展密切相关．
Objective: To establish a model of rat brain abscess and explore the relationship between changes of ATPase activity and brain edema in abscess tissue. Methods: The right frontal lobe of rats was injected with bacterial suspension (1μl, 104 Staphylococcus aureus). The activity of ATPase and water content in the brain tissue around abscess were measured 1 ~ 19 days after inoculation. RESULTS: After inoculation of bacteria, the rats developed abscess in the right frontal lobe. The pathological process was similar to the clinical situation. The ATPase activity in the surrounding tissue of abscess decreased persistently (within 7 days), while the water content increased progressively. There was a highly negative correlation between cerebral edema and changes of the activity of Na + K + -ATPase (r = -0.865) and Ca2 + ATPase (r = -0.881) (P <0.01). CONCLUSION: Quantitative bacterial colonization in specific parts of rat brain can stably replicate brain abscess model. The inhibition of ATPase activity in the surrounding tissue of abscess is closely related to the occurrence and development of cerebral edema.