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目的 建立用高效毛细管电泳仪拆分手性药物中间体苯基乙二醇、苯基环氧乙烷的方法。方法 利用磺酰化 β 环糊精作为手性选择剂 ,磷酸 三乙胺为电泳缓冲液 ,电压为 2 0kV ,毛细管柱温度为 18℃ ,检测波长为 2 0 0nm ,石英毛细管柱有效长度 2 7cm ,采用反极性模式对两化合物进行分离。研究缓冲溶液pH值、环糊精浓度、有机溶剂添加剂等因素对分离度的影响。结果 苯基乙二醇、苯基环氧乙烷均获得基线分离 ,并对其绝对构型进行了确认。得到了最佳的分离条件。结论 该方法简单、快速 ,分离度、重现性良好。对用磺酰化β 环糊精分离中性化合物具有重要意义。
OBJECTIVE To establish a method for the separation of phenylglycol and phenyloxirane from chiral drugs by high performance capillary electrophoresis. Methods Sulfonylated β-cyclodextrin was used as chiral selector. Triethylamine phosphate was used as electrophoresis buffer. The voltage was 20 kV, the temperature of capillary column was 18 ℃, the detection wavelength was 200 nm, the effective length of quartz capillary column was 27 cm , The reverse polarity mode was used to separate the two compounds. The influence of buffer pH value, cyclodextrin concentration and organic solvent additives on the resolution was studied. Results Both phenylglycol and phenylene oxide were baseline separated and the absolute configuration was confirmed. Get the best separation conditions. Conclusion The method is simple, rapid, accurate and reproducible. Separation of neutral compounds with sulfonylated β-cyclodextrin is of great importance.