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干旱胁迫条件下考察转AtCBF4基因大豆株系的光合特性(净光合速率、气孔导度、胞间CO2浓度、蒸腾速率、瞬时水分利用率)、生理特性(脯氨酸、丙二醛、可溶性糖)以及产量性状(株高、节数、单株荚数、单株粒数、百粒重)的表现,综合评价7个株系之间的抗旱性差别。结果表明:转AtCBF4基因大豆叶肉细胞中脯氨酸的含量和可溶性糖含量与受体相比较高,丙二醛含量增幅较小,光合生产能力和产量性状表现优于受体。综合结荚期和鼓粒期表现,所有转基因株系耐旱性均强于非转基因受体,其中HTCB59-5、HTCB59-2和HTCB59-4具有较强的耐旱能力。
Under drought stress, the photosynthetic characteristics (photosynthetic rate, stomatal conductance, intracellular CO2 concentration, transpiration rate, instantaneous water use efficiency), physiological characteristics (proline, malondialdehyde, soluble sugar ) And yield traits (plant height, number of sections, number of pods per plant, number of grains per plant, and weight of 100 grains), and comprehensively evaluated the differences in drought resistance among the seven lines. The results showed that the contents of proline and soluble sugar in soybean leaf cells transfected with AtCBF4 gene were higher than that of the receptor and the content of malondialdehyde increased less, and photosynthetic capacity and yield traits performed better than the receptor. Comprehensive pod stage and drum stage showed that all the transgenic lines had stronger drought tolerance than non-transgenic lines. Among them, HTCB59-5, HTCB59-2 and HTCB59-4 had stronger drought tolerance.