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用根癌农杆菌感染芥菜型油菜子叶外植体，培养２～３ｄ，然后转到含Ｋｍ１０～２０ｍｇ／Ｌ的选择培养基上筛选转化体，在选择培养基上获得的Ｋｍ抗性苗，进一步在含Ｋｍ３０～５０ｍｇ／Ｌ的选择培养基上筛选３～４月，然后诱导生根，再生成完整植株．ＤＮＡ分子杂交证明Ｂ．ｔ．基因已整合到油菜基因组中，转化频率为１％～２％．
The Agrobacterium tumefaciens was used to infect the Brassica campestris cotyledon explants and cultured for 2 to 3 days. Then, the transformants were selected on the selective medium containing Km10 ~ 20mg / L, and the Km resistant seedlings obtained on the selection medium were further cultured The selection medium containing Km30 ~ 50mg / L screening 3 to 4 months, and then induce rooting and regeneration of the entire plant. DNA molecular hybridization certificate t. The gene has been integrated into the genome of rape and the transformation frequency is 1% -2%.