论文部分内容阅读
1目的 探讨慢性肝病病人外周血中一氧化氮 (NO)和肿瘤坏死因子 α(TNF- α)含量的变化及其意义。 2方法 采用 Griess法和双抗体夹心 EL ISA法测定 11例慢性迁延性肝炎 (CPH)、12例慢性活动性肝炎(CAH)、17例肝硬化 (L C)病人外周血中 NO和 TNF- α的含量 ,并与 33例正常人进行了比较。 3结果 慢性肝病病人血清中 TNF- α与 NO明显高于对照组 (t=2 .377～ 7.0 74,P<0 .0 5 ) ,且 TNF- α与 NO呈正相关 (r=0 .811,P<0 .0 1)。 4结论 TNF- α参与了肝脏的活动性病变过程 ,NO对病毒性肝炎肝细胞有损伤作用
1 Objective To investigate the changes and significance of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) in peripheral blood of patients with chronic liver disease. Methods The levels of NO and TNF-α in peripheral blood of 11 patients with chronic persistent hepatitis (CPH), 12 patients with chronic active hepatitis (CAH) and 17 patients with liver cirrhosis (LC) were measured by Griess method and double antibody sandwich ELISA. Content, and with 33 normal subjects were compared. 3 Results Serum levels of TNF-α and NO in patients with chronic liver disease were significantly higher than those in the control group (t = 2.777 ± 7.074, P <0.05), and there was a positive correlation between TNF- α and NO (r = 0.81 1, P <0 .0 1). 4 Conclusion TNF-α involved in the process of active lesions of the liver, NO of hepatitis virus-induced liver injury