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目的:建立适用于开展人参栽培土壤微生物种群结构分析的ARDRA研究体系。方法:从基因组水平研究人参栽培土壤微生物种群多样性结构。结果:对人参栽培土壤样品分析发现,ARDRA体系不仅能高效区分土壤中不同的微生物类群,而且还可根据酶切谱带的差异统计出不同微生物类群在土壤总微生物中所占的比重。结论:建立的ARDRA研究体系能够用于人参栽培土壤微生物种群结构分析。
OBJECTIVE: To establish an ARDRA research system suitable for the analysis of soil microbial structure in cultivated ginseng. Methods: To study the diversity of microbial population in ginseng cultivated soils from the genomic level. Results: Analysis of ginseng cultivated soil samples showed that the ARDRA system not only can effectively distinguish different microbial groups in the soil, but also can calculate the proportion of different microbial groups in the total soil microorganisms according to the difference of the digested bands. Conclusion: The established ARDRA system can be used to analyze the population structure of ginseng cultivated soils.