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建立了反相高效液相色谱法测定对乙酰氨基酚血浆中浓度的方法。方法使用YWGC18柱（4．6×250mm，10μm）；流动相为甲醇：醋酸－醋酸钠：二乙胺（20：80：0．5）；以茶碱为内标物；血清处理用甲醇沉淀蛋白；紫外检测波长244nm；流动相流速1．5ml／min；最低检出浓度0．05μg／ml。对乙酰氨基酚血药浓度在0．25~25μg／ml范围内线性关系良好（r＝0．9999）。本方法适用于对乙酸氨基酚的血药浓度测定。
A method was developed for the determination of paracetamol in plasma by reversed-phase high performance liquid chromatography. The method used YWGC18 column (4.6 × 250mm, 10μm); the mobile phase was methanol: acetic acid-sodium acetate: diethylamine (20:80:50); theophylline was used as internal standard; Protein; UV detection wavelength 244nm; mobile phase flow rate 1.5ml / min; minimum detectable concentration 0.05μg / ml. Paracetamol plasma concentrations in the 0.25 ~ 25μg / ml range of good linear relationship (r = 0.9999). This method is suitable for the determination of plasma concentration of acetaminophen.