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以前的研究表明,正常胎盘个体间15-羟-前列腺素脱氢酶(PGDH)活性差异很大,且这种差异与妊娠的临床特征无任何相关性,原因尚不清楚。为确定控制胎盘 PGDH 活性究竟是母体还是胎儿占主导地位,作者测定了来自遗传学相同和不同的多胎妊娠胎盘中 PGDH 活性。实验所用胎盘来自15个双胎和1个三胎,经自然阴道分娩后立即取到,置于冰上。在胎盘的脐带至外边缘之间选取4个绒毛叶,重1～2g。迅速在0.9%的冰盐水中清洗,吸干水份,立即冷冻于液氮中。从分娩完成到冷冻的时间一般不超过30′。分娩后两周内,冷冻样本称重、切片、匀浆化,然后与饱和量 PGF_(2α)一起孵化,后用氯仿提取,薄层色谱分离 PGF_(2α)及其代谢产物。以回收的 PGF_(2α)与其代谢产物比率的线性回归来计算 PGDH 活性。用 t
Previous studies have shown that the activity of 15-hydroxy-prostaglandin dehydrogenase (PGDH) varies widely among individuals with normal placenta and that this difference has no correlation with the clinical features of pregnancy, for reasons not yet known. To determine whether maternal or fetal predominance controls PGDH activity in the placenta, the authors determined PGDH activity in placentas of the same and different gestations of gestation. The placenta used in the experiment was from 15 twins and one third child and was taken immediately after natural vaginal delivery and placed on ice. In the placenta between the outer edge of the umbilical cord selected 4 fluff, weighing 1 ~ 2g. Rinse quickly in 0.9% ice saline, blot dry and immediately freeze in liquid nitrogen. From birth to freezing time is generally not more than 30 ’. Frozen samples were weighed, sliced and homogenized within two weeks after delivery, then hatched with saturated PGF 2α and extracted with chloroform. The PGF 2α and its metabolites were separated by thin-layer chromatography. PGDH activity was calculated as a linear regression of the ratio of PGF_ (2α) recovered to its metabolites. With t