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[Objective] To conduct simultaneous determination of selenium and germanium content in traditional Chinese medicine,such as wild RHIZOMA GASTRODIAE,RADIX POLYGONI MULTIFLORI,RADIX ANGELICAE SINENSIS,RADIX ADENOPHORAE,and RHIZOMA COPTIDIS,by hydride generation-atomic fluorescence spectroscopy. [Methods] The samples were prepared by nitric acid-hydrogen peroxide microwave digestion,using phosphoric acid as the medium and thiourea as the pre-reductant,the selenium and germanium contents of traditional Chinese medicine were determined by dual channel atomic fluorescence spectrometry. [Results] Under the optimized conditions,the instrumental detection limits of selenium and germanium were 0. 25 and 1. 46 ng / m L,and the RSDs were 1. 9%( 5 ng / m L,n = 10) and2. 9%( 10 ng / m L,n = 10),and the recoveries were 91. 7%- 97. 2% and 89. 5%- 95. 7% with RSD less than 4%( n = 10),respectively.The linear ranges were all 0- 300 ng / m L. Interference ions as Cr3 +,Cu2 +,Zn2 +and Co2 +had the biggest influences on selenium,while Pb2 +and Ni2 +the biggest influences on germanium. These interference ions were not considered,because their contents were not high enough to disturb selenium and germanium determination. [Conclusions]The selenium contents of 5 kinds of medicinal materials were in the range of533. 1- 1 773 ng / g,the order was: wild RHIZOMA GASTRODIAE > RHIZOMA COPTIDIS > RADIX POLYGONI MULTIFLORI > RADIX ANGELICAE SINENSIS > RADIX ADENOPHORAE; the germanium content range was 253. 9- 842. 5 ng / g,the order was: wild RHIZOMA GASTRODIAE > RADIX ADENOPHORAE > RADIX ANGELICAE SINENSIS > RADIX POLYGONI MULTIFLORI > RHIZOMA COPTIDIS. Two element contents of wild RHIZOMA GASTRODIAE were higher than that of other cultivated samples.
[Objective] To conduct simultaneous determination of selenium and germanium content in traditional Chinese medicine, such as wild RHIZOMA GASTRODIAE, RADIX POLYGONI MULTIFLORI, RADIX ANGELICAE SINENSIS, RADIX ADENOPHORAE, and RHIZOMA COPTIDIS, by hydride generation-atomic fluorescence spectroscopy. [Methods] The samples were prepared by nitric acid-hydrogen peroxide microwave digestion, using phosphoric acid as the medium and thiourea as the pre-reductant, the selenium and germanium contents of traditional Chinese medicine were determined by dual channel atomic fluorescence spectrometry. [Results] Under the optimized conditions, the instrumental detection limits of selenium and germanium were 0. 25 and 1. 46 ng / m L, and the RSDs were 1.9% (5 ng / m L, n = 10) and 2. 9% (10 ng / m L, n = 10), and the recoveries were 91. 7% -97.2% and 89.5% -95.7% with RSD less than 4% (n = 10), respectively. 0-300 ng / m L. Interference ions as Cr3 +, Cu2 +, Zn2 + and Co2 + had the biggest influences on selenium, while Pb2 + and Ni2 + the biggest influences on germanium. These interference ions were not considered, because their contents were not high enough to disturb selenium and germanium determination. [Conclusions] The selenium contents of 5 kinds of medicinal materials were in the range of 533. 1- 1 773 ng / g, the order was: wild RHIZOMA GASTRODIAE> RHIZOMA COPTIDIS> RADIX POLYGONI MULTIFLORI> RADIX ANGELICAE SINENSIS> RADIX ADENOPHORAE; the germanium content range was 253. 9-84 2. 5 ng / g , the order was: wild RHIZOMA GASTRODIAE> RADIX ADENOPHORAE> RADIX ANGELICAE SINENSIS> RADIX POLYGONI MULTIFLORI> RHIZOMA COPTIDIS. Two element contents of wild RHIZOMA GASTRODIAE were higher than that of other cultivated samples.