论文部分内容阅读
建立了基于离子液体的原位溶剂形成微萃取-高效液相测定虾肉中氯霉素的新方法。考察并优化了基于离子液体的原位溶剂形成微萃取虾肉中氯霉素的条件,包括离子液体的种类与用量、离子交换剂的种类与用量、微波条件、涡旋震荡时间等。优选最佳萃取条件为:600μL离子液体[C_6MIN][BF_4]作萃取剂,4000μL LiNTF_2作为离子交换试剂,无需微波辐射,旋涡震荡10min,此条件下,虾肉中氯霉素的回收率为92.5%~98.3%,相对标准偏差为3.7%~5.9%,检出限为5.8μg/kg。该法简便、快速、环保。
A new method for the determination of chloramphenicol in shrimp meat by in situ solvent-based micro-extraction and high performance liquid phase based on ionic liquids was established. The conditions for the formation of chloramphenicol in shrimp by in-situ solvent-based ionic liquids were investigated and optimized, including the types and amounts of ionic liquids, the types and amounts of ion exchangers, microwave conditions, vortex oscillation time and so on. The optimal extraction conditions were as follows: 600μL ionic liquid [C_6MIN] [BF_4] as extractant and 4000μL LiNTF_2 as ion exchange reagent without vortexing for 10min. Under these conditions, the recovery of chloramphenicol in shrimp meat was 92.5 % ~ 98.3%, the relative standard deviation was 3.7% ~ 5.9%, the detection limit was 5.8μg / kg. The method is simple, fast and environmentally friendly.