应用反向间接血凝法检测水稻白叶枯病病原

• 作 者: 王法明 陈毓苓 邓秀蓉 张光洛 殷尚智 奚文英
• 机 构: 江苏省农科院植保所,江苏省农科院植保所,江苏省农科院植保所,江苏省农科院植保所,江苏省农科院植保所,江苏省农科院植保所
• 出 处: 中国农业科学
• 发表日期: 1980年02期
• 关键词: 反向间接血凝法 水稻白叶枯病 水稻白叶枯病菌 病原细菌 噬菌体 分离培养法 镜检观察 接种法 稻桩 白叶枯病

防治水稻白叶枯病,目前虽有一套行之有效的综合防治措施,但由于对病原细菌侵染规律摸得不透,因而在措施上环环都要抓,手续繁琐,急需进一步提高。目前检测水稻白叶枯病菌常用的方法主要有分离培养,噬菌体法、镜检观察喷菌法、浓缩接种法等数种,均有一定的缺点。如分离培养法容易污染,而且标样一经干燥即不易检出;噬菌体

At present, although there is an effective set of comprehensive prevention and control measures, due to the impermeable infection of pathogenic bacteria, the ring should be grasped on the measures and the procedures are cumbersome and need to be further improved urgently. At present, the common methods for detecting bacterial leaf blight of rice are mainly isolation and culture, bacteriophage method, microscopic examination of bacterial spray, and concentrated inoculation method, all of which have certain disadvantages. Such as separation and culture method is easy to contaminate, and the standard sample is not easy to detect after drying; phage