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目的 :探讨米非司酮对人卵巢上皮性癌细胞的杀伤活性及对细胞增殖和细胞周期分布的影响。方法 :采用MTT测定 ,观察不同浓度米非司酮 (2 0、10、5、2 5 μmol/L)对HO - 8910细胞的杀伤活性 ;通过ABC免疫组化染色及流式细胞仪分析 ,检测用药后HO - 8910细胞Ki- 6 7抗原表达、细胞周期分布及细胞增殖指数的变化。结果 :4个浓度的米非司酮对HO - 8910细胞均有杀伤作用 ,并呈剂量依赖性 ,以 2 0 μmol/L浓度杀伤作用最明显 ,杀伤率达 5 2 0 1% ;5 μmol/L米非司酮可使HO - 8910细胞Ki- 6 7抗原表达显著减少 ,细胞增殖受到明显抑制 ,使细胞阻滞于G0 /G1期 ,不能进入S及G2 /M期 ,细胞增殖指数明显下降。结论 :米非司酮体外对人卵巢上皮性癌细胞具有杀伤活性 ,并对细胞增殖及细胞周期有明显抑制作用 ,主要通过抑制DNA合成 ,将细胞阻滞于G0 /G1期。
Objective: To investigate the effects of mifepristone on human ovarian epithelial carcinoma cells and their effects on cell proliferation and cell cycle distribution. Methods: MTT assay was used to observe the cytotoxicity of mifepristone (2 0, 10, 5, 25 μmol / L) on HO - 8910 cells. ABC immunohistochemical staining and flow cytometry Changes of Ki67 antigen expression, cell cycle distribution and cell proliferation index in HO - 8910 cells after treatment. Results: Four concentrations of mifepristone could kill HO - 8910 cells in a dose - dependent manner. The killing effect was most pronounced at a concentration of 20 μmol / L, with a killing rate of 52.01%. 5 μmol / L Mifepristone could significantly decrease the expression of Ki- 6 7 antigen in HO - 8910 cells and significantly inhibit the cell proliferation in G0 / G1 phase, fail to enter S and G2 / M phase and significantly decrease the cell proliferation index . CONCLUSION: Mifepristone has cytotoxic activity on human ovarian epithelial carcinoma cells in vitro and significantly inhibits the proliferation and cell cycle of the human ovarian epithelial cancer cells. The inhibition of DNA synthesis inhibits the cell cycle arrest at G0 / G1 phase.