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为存留抗乳腺癌人单抗CM－1的可变区编码基因，从CM-1杂交瘤细胞中提出总RNA，经逆转录生成cDNA，PCR后得到一400hP左右的基因扩增片段。用一条内引物直接对PCR产物进行基因序列测定，测得344个碱基序列，与已知人抗体重链可变区的读码框架相符，其对应的氨基酸序列属人抗体可变区“典范结构”的1～3类，从而进一步验证了CM-1单抗的人源性，并为人抗体编码基因的研究提供了新资料，也为制备CM－1小分子抗体作了准备。
To retain the gene encoding the variable region of CM-1, a human anti-breast cancer human monoclonal antibody, total RNA was extracted from CM-1 hybridoma cells and cDNA was generated by reverse transcription. After PCR, a gene amplification fragment of about 400 hP was obtained. The PCR product was directly sequenced with an internal primer and the 344 base sequence was obtained, which is consistent with the known human antibody heavy chain variable region frame. The corresponding amino acid sequence is a variable region of the human antibody. "1 to 3 categories, which further verify the human origin of CM-1 monoclonal antibody, and provide new information for the study of human antibody encoding genes, but also prepared for the preparation of CM-1 small molecule antibodies.