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采用链霉菌抗生物素蛋白—过氧化物酶连接法（Ｓ－Ｐ法），研究Ｐ１６蛋白在胃癌和癌前病变（不典型增生、慢性萎缩性胃炎及慢性消化性胃溃疡）中的表达。结果显示，Ｐ１６蛋白阳性表达：胃癌４７．５４％（５８／１２２），癌前病变９０．００％（７２／８０），正常胃粘膜９６．２５％（７７／８０），高分化腺癌３７．９３％（１１／２９），低分化腺癌５１．２２％（２１／４１），未分化癌５７．６９％（１５／２６），印戒细胞癌６２．５０％（１０／１６），粘液腺癌１０．００％（１／１０）。经统计学处理，胃癌组与正常对照组及癌前病变组间的差异均有显著性意义（Ｐ＜０．０５）；粘液腺癌与低分化腺癌、未分化腺癌及印戒细胞癌之间的差异也均有显著性意义（Ｐ＜０．０５）。
Streptavidin-peroxidase ligation (S-P method) was used to study the expression of P16 protein in gastric cancer and precancerous lesions (atypical hyperplasia, chronic atrophic gastritis, and chronic peptic gastric ulcer). The results showed that the positive expression of P16 protein: gastric cancer 47.54% (58/122), precancerous lesion 90.00% (72/80), normal gastric mucosa 96.25% (77/80), well-differentiated adenocarcinoma 37 .93% (11/29), 51.22% (21/41) of poorly differentiated adenocarcinoma, 57.69% (15/26) of undifferentiated carcinoma, 62.50% (10/16) of signet ring cell carcinoma, Mucinous adenocarcinoma 10.00% (1/10). After statistical analysis, there were significant differences between the gastric cancer group and the normal control group and precancerous lesion group (P<0.05); mucinous adenocarcinoma and poorly differentiated adenocarcinoma, undifferentiated adenocarcinoma, and signet ring cell carcinoma The differences between the two groups were also significant (P<0.05).