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以三聚氰胺甲醛(MF)微粒为模板,采用逐层静电自组装技术交替吸附聚苯乙烯磺酸钠(PSS)和聚烯丙基胺盐酸盐(PAH),得到具有核壳结构的复合式微球,然后通过pH=1的盐酸溶液除去中心模板,得到直径约为3～4μm的空腔胶囊.使用藻红蛋白作为探针分子,通过比较空腔胶囊装载前后荧光强度的变化,发现pH在4～5之间时,胶囊呈现最大的蛋白装载量.pH在6～10的范围内,藻红蛋白在胶囊上的装载量几乎不变.pH<3时,装载能力很差.此外,通过荧光共聚焦显微镜对不同pH条件下的蛋白装载规律进行了成像分析.一部分藻红蛋白在pH=4的条件下通过扩散进入了胶囊的内部,而pH=7的条件下,藻红蛋白不进入胶囊内部,而是吸附在表面.
Melamine-formaldehyde (MF) microparticles were used as templates, and PSS and polyallylamine hydrochloride (PAH) were alternately adsorbed by layer-by-layer electrostatic self-assembly technique to obtain composite microspheres with core- , And then the center template was removed by using hydrochloric acid solution of pH = 1 to obtain a hollow capsule with a diameter of about 3 to 4 μm. Using phycoerythrin as a probe molecule, the change of the fluorescence intensity before and after loading of the cavity capsule was found, ~ 5, the capsule showed the largest protein loading.PH was almost unchanged in the range of 6 ~ 10, the loading of phycoerythrin on the capsule was very poor, and the loading ability was poor at pH <3. In addition, Confocal microscopy images of protein loading under different pH conditions were carried out.A portion of phycoerythrin entered the capsule by diffusion at pH = 4, whereas phycoerythrin did not enter the capsule at pH = 7 Internal, but adsorbed on the surface.