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目的探讨肝组织间粘附分子-1(ICAM-1)表达在慢性乙型肝炎(CHB)发病机理中的作用。方法用原位杂交和免疫组织化学技术检测11例正常人和50例慢性HBV感染者肝内ICAM-1 mRNA和ICAM-1表达情况。结果正常人和慢性无症状HBsAg携带者肝细胞无ICAM-1mRNA和ICAM-1表达,CHB患者肝细胞ICAM-1 mRNA和ICAM-1表达增强,阳性肝细胞多分布在汇管区周围和腺泡内炎症坏死区域;重度CHB患者肝细胞ICAM-1 mRNA和ICAM-1表达显著强于中、轻度CHB患者(P<0.05);肝细胞ICAM-1表达强度与肝组织炎症活动度呈显著正相关,p<0.01;肝细胞ICAM-1表达强的患者肝功能显著差于ICAM-1表达弱者,P<0.05。结论肝细胞ICAM-1表达在慢性乙型肝炎肝细胞坏死中起重要作用,肝细胞ICAM-1表达水平能较好反映其肝损害程度和肝功能状况。
Objective To investigate the role of intercellular adhesion molecule-1 (ICAM-1) expression in the pathogenesis of chronic hepatitis B (CHB). Methods In situ hybridization and immunohistochemistry were used to detect ICAM-1 mRNA and ICAM-1 expression in 11 normal and 50 chronic HBV infected patients. Results There was no expression of ICAM-1 mRNA and ICAM-1 in normal and chronic asymptomatic HBsAg carriers, and the expression of ICAM-1 mRNA and ICAM-1 in hepatocytes of CHB patients were increased. Positive hepatocytes were distributed around the portal area and in the acinus The expression of ICAM-1 mRNA and ICAM-1 in hepatocytes in severe CHB patients was significantly higher than that in moderate and mild CHB patients (P <0.05). The expression intensity of ICAM-1 in hepatocytes was positively correlated with the inflammation activity of liver tissue , p <0.01. The liver function in patients with strong expression of ICAM-1 in liver cells was significantly lower than that in ICAM-1 patients with poor expression, P <0.05. Conclusion The expression of ICAM-1 in hepatocytes plays an important role in the hepatocyte necrosis in chronic hepatitis B. The ICAM-1 expression in hepatocytes can better reflect the extent of liver damage and liver function.