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目的:建立以人参皂苷Re为内参物,同时测定藤珠胃康颗粒中4个活性成分(人参皂苷Re、人参皂苷Rb1、人参皂苷Ro和竹节参皂苷Ⅳa)的一测多评法。方法:以人参皂苷Re为内参物,建立人参皂苷Rb1、人参皂苷Ro和竹节参皂苷Ⅳa 3个成分相对于人参皂苷Re的相对校正因子。采用外标法测定藤珠胃康颗粒中人参皂苷Re的含量,通过相对校正因子对其他3个成分进行定量分析,并将该方法的测定结果与外标法的测定结果相比较。结果:各成分相对校正因子重现性良好。人参皂苷Re、人参皂苷Rb1、人参皂苷Ro和竹节参皂苷Ⅳa按一测多评方法与外标法测定结果无显著差异。结论:本文建立的方法经方法学验证,可为控制藤珠胃康颗粒的内在质量提供参考。
OBJECTIVE: To establish a multiplex assay for the determination of 4 active ingredients (ginsenoside Re, ginsenoside Rb1, ginsenoside Ro and sjgadsenoside Ⅳa) with ginsenoside Re as internal reference substance and simultaneously with the determination of ginsenoside. Methods: Ginsenoside Re was used as internal reference to establish the relative correction factors of ginsenoside Rb1, ginsenoside Ro and sanshinone Ⅳa relative to ginsenoside Re. The content of ginsenoside Re in Tengzhuweikang Granule was determined by external standard method. The other three components were quantitatively analyzed by relative correction factor, and the results of this method were compared with those of external standard method. Results: The relative correction factor of each component has good reproducibility. Ginsenoside Re, ginsenoside Rb1, ginsenoside Ro and ginseng saponin Ⅳa according to a test multiple evaluation method and the external standard test results no significant difference. Conclusion: The method established in this paper is validated by methodology, which can provide reference for controlling the intrinsic quality of Teng Chu Wei Kang Granules.