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目的:观察山羊乳牙牙髓细胞(SGDs)与多孔磷酸钙骨水泥之间的生物相容性及其异位成骨能力。方法:流式细胞仪检测第1代SGDs的STRO-1表达情况;体外培养的第4代SGDs进行成骨诱导7d后,与多孔磷酸钙骨水泥复合培养,扫描电镜下观察SGDs黏附和生长情况;细胞材料复合物植入裸鼠皮下8周观察异位成骨能力。结果:SGDs与多孔磷酸钙骨水泥复合培养3d后,细胞与材料表面贴合,形态正常,可见细胞伸出伪足,分泌胞外基质;裸鼠体内植入8周后,HE染色显示骨样组织形成,免疫组化OC呈阳性表达。结论:SGDs可以向成骨细胞分化,并具有诱导成骨能力,与多孔磷酸钙骨水泥结合,可形成骨样组织。
Objective: To observe the biocompatibility and ectopic osteogenesis of goat primary dental pulp cells (SGDs) and porous calcium phosphate cement. Methods: The expression of STRO-1 in the first generation of SGDs was detected by flow cytometry. The cultured SGs of the fourth generation were cultured with calcium phosphate cement after 7 days of osteogenic induction. The adhesion and growth of SGDs were observed under scanning electron microscope Cell material composite implanted into nude mice subcutaneously for 8 weeks to observe the ability of ectopic osteogenesis. Results: After 3d cells were incubated with porous calcium phosphate cement for three days, the cells adhered to the surface of the material with normal morphology. The cells extended pseudopodia and secreted the extracellular matrix. After 8 weeks implantation in nude mice, HE staining showed osteoid Tissue formation, immunohistochemistry OC was positive. CONCLUSIONS: SGDs can differentiate into osteoblasts and induce osteogenesis. Combined with porous calcium phosphate cement, SGDs can form osteoid tissue.