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目的通过研究NF-κВ在DM/PM患者骨骼肌的表达情况,以及DM患者血清体外对脐静脉内皮细胞(HUVEC)NF-κВ表达的影响,初步探讨NF-κВ在DM/PM中的相关作用的机制。方法采用免疫组织化法对25例DM/PM患者骨骼肌中的NF-κВ(P65)进行检测,取16例DM患者治疗前和6例治疗后的血清加入体外培养的HUVEC中,用细胞免疫组织化法检测HUVEC株NF-κВ(P65)的表达。结果 NF-κВ(P65)在DM/PM患者骨骼肌内的微血管内皮细胞和肌纤维中表达上调,与正常对照组相比差异有显著性(P<0.05),正常对照组无表达;DM患者治疗前血清体外作用HUVEC 6h后,(11.126±1.920)%细胞表达阳性,24h后细胞的阳性率上升到(17.048±1.140)%,差异有统计学意义(P<0.01);治疗后血清对脐静脉内皮细胞(HUVEC)的NF-KB(P65)阳性表达率分别为(6.797±0.609)%(6h)和(11.273±0.643)%(24h),二者均低于相应的治疗前表达率(P<0.01)。结论 NF-κВ在DM/PM肌肉微血管肌纤维的炎症性反应过程中发挥重要作用,其在DM患者血清中可能有激活NF-κВ的成分。
OBJECTIVE: To investigate the effect of NF-κB in the skeletal muscle of DM / PM patients and the expression of NF-κВ in human umbilical vein endothelial cells (HUVEC) Mechanisms. Methods Immunohistochemical method was used to detect NF-κB (P65) in skeletal muscle of 25 patients with DM / PM. Sixteen DM patients were treated with the serum before and after the treatment, and 6 serums were added to the cultured HUVEC. HUVEC strains NF-κВ (P65) expression was detected by histochemistry. Results The expression of NF-κB (P65) was up-regulated in the microvascular endothelial cells and myofibers of skeletal muscle in DM / PM patients. Compared with the normal control group, the expression of NF-κB was not significantly different (P <0.05) The positive rate of HUVEC in vitro was (11.126 ± 1.920)% after 6 h, and the positive rate of HUVEC increased to (17.048 ± 1.140)% after 24 h, the difference was statistically significant (P <0.01) The positive rates of NF-κB P65 in HUVEC were (6.797 ± 0.609)% (6h) and (11.273 ± 0.643)% (24h) respectively, both of which were lower than the corresponding pre-treatment rates <0.01). Conclusion NF-κВ plays an important role in the inflammatory reaction of DM / PM muscle microvascular muscle fibers, which may activate NF-κB in serum of DM patients.