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Objective:To exploretheserum-freecultureconditionsfordifferentiatingmouseembryonicstemcells(ES cells)intoneuralprecursorcells(NPC)andcomparetheeffectsof humanembryonicfibroblasts(HEF)as thefeederlayer of ES withthatof mouseembryonicfibroblasts(MEF)in vitro.Methods:MouseES cellswereculturedin or notin feederlayer cellsmediumcontainingor notleukemiainhibitoryfactorto suppresstheirdifferentiation.Immunocytochemicalmethod was usedto identifyNPCby detectingnestinantigenandalkalinephosphatase.Results: TheES cellsculturedin HEF werepositiveto alkalinephosphatase.Serum-freemediumallowedthedifferentiationof ES cellsintoNPC.Conclusion:HEFcouldreplaceMEFandkeeptheundifferentiatedconditionof ES cellswithmorebenefits.NPCof highpuritycould be culturedfromEScellsby serum-freeculturemethod.
Objective: To exploretheserum-freecultureconditionsfordifferentiatingmouseembryonicstemcells (ES cells) intoneuralprecursorcells (NPC) andcomparetheeffectsof humanembryonicfibroblasts (HEF) as thefeederlayer of ES withthatof mouseembryonicfibroblasts (MEF) in vitro.Methods: MouseES cellswereculturedin or notin feederlayer cellsmediumcontainingor notleukemiainhibitoryfactorto suppresstheirdifferentiation.Immunocytochemicalmethod was usedto identifyNPCby detectingnestinantigenandalkalinephosphatase.Results: TheEScellsculturedin HEF werepositiveto alkalinephosphatase.Serum-freemediumallowedthedifferentiationofEEScellsintoNPC.Conclusion: HEFcouldreplaceMEFandkeptofUnifferentiatedconditionofEES cellswithmorebenefits .NPCofhighpuritycouldbefulturedfromEScellsby serum-free culturemethod.