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建立了一种测定人血浆中甲磺酸培氟沙星的匠相液相色谱方法，用的是ＺｏｒｂａｘＯＤＳＣ＿（１８）柱和用三乙醇胺调节成ｐＨ为３．０的甲醇－０．０４ｍｏｌ／ＬＨ＿３ＰＯ＿４溶液（８０：２０，Ｖ／Ｖ）的流动相，检测在２７６ｎｍ处进行，流量为１ｍＬ／ｍｉｎ，线性范围是６．０１×１０￣（－８）～３．０１×１０￣（－５）ｍｏｌ／Ｌ（γ＝０．９９９８），检测极限为６．０１×１０￣（－８）ｍｏｌ／Ｌ，平均回收率为９７．５７±３．０４％，日内与日间的ＣＶ分别为３．２９％和５．０８％。
A phase-HPLC method was developed for the determination of pefloxacin mesylate in human plasma using a Zorbax ODSC 18 column and a methanol-0.04 mol / LH 3 PO 4 solution adjusted to pH 3.0 with triethanolamine The mobile phase of the solution (80:20, V / V) was detected at 276 nm at a flow rate of 1 mL / min with a linear range of 6.01 × 10 -8 to 3.01 × 10 -5. mol / L (γ = 0.9998). The detection limit was 6.01 × 10 ~ (-8) mol / L and the average recovery was 97.57 ± 3.04%. The daily and intraday CVs were 3 .29% and 5.08%.