论文部分内容阅读
以籼型两用不育系水稻培矮645和常规稻粳籼89为材料，采用PAGE分析同工酶的方法，比较水稻幼穗发育过程中花粉母细胞形成期、减数分裂期和花粉完熟期NAD＋-MDH和AP同工酶的变化，以期了解不育系的育性转换与表达机理。结果表明，在1993年2月25日、3月10日和3月30日三期播种的水稻培矮645均表现不育，花粉不育度在99.7％以上，结实率在0.3％以下，1994年8月1日播种的培矮645表现可育，结实率在19％以上，而同期播种的常规稻粳籼89均能得到正常结实，结实率在68％以上。同时发现1993年培矮645的不育性在花粉完熟期与颖花及花药的NAD＋-MDH活性的显著降低关系较大。在花粉母细胞形成期一减数分裂期与颖花AP的活性及同工酶组成的变化关系较密切。1994年表现可育的培矮645与粳籼89相比不存在这种差异。由此可认为培矮645育性表达可能与花粉发育前期的脂肪代谢以及花粉发育后期的呼吸代谢有关。
In this study, the isogenic lines of indica type CMS lines Pei’ai 645 and conventional rice japonica rice 89 were used to analyze the formation of pollen mother cells, meiosis and pollen maturation Phase NAD + -MDH and AP isozymes changes in order to understand the sterile line of fertility conversion and expression mechanism. The results showed that Pei’ai 645, sowed on March 25, March 10 and March 30, 1993 all showed infertility, pollen sterility above 99.7%, seed setting rate below 0.3%, 1994 Pei’ai 645, sown on August 1, was fertile with a seed setting rate above 19%. Normal rice japonica rice sown in the same period all achieved normal seed setting with a seed setting rate above 68%. At the same time, it was found that Pei’ai 645 infertility in 1993 had a significant relationship with the significant decrease of NAD + -MDH activity in spikelets and anther at the pollen ripening stage. Pollen mother cells in the formation of a meiosis stage and spike AP activity and isozyme composition changes are closely related. There was no such difference between Pei’ai 645 which is fertile in 1994 and Japonica 89. Therefore, we can conclude that the fertility expression of Pei’ai 645 may be related to the lipid metabolism in the pre-pollen stage and the respiratory metabolism in the late pollen stage.