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目的:探讨IGF-1和IGFBP-3在结直肠癌(colorectal cancer,CRC)组织中的表达及其临床意义。方法:选取承德医学院附属保定市第一中心医院2005-06-13-2012-12-27接受手术治疗的CRC患者183例作为观察组,并对其中61例取其癌旁正常黏膜组织作为对照组,应用免疫组化SP法分别检测IGF-1和IGFBP-3在两组的表达情况。结果:IGF-1在CRC中的阳性表达率60.1%(110/183),显著高于对照组31.1%(19/61),P<0.001;IGFBP-3在CRC中的阳性表达率78.1%(143/183),显著低于对照组95.1%(58/61),P=0.003。IGF-1表达与组织学分级、浸润深度、TNM分期和淋巴结转移有关,P<0.05;IGFBP-3表达与TNM分期和淋巴结转移有关,P<0.05;两者与年龄、性别、肿瘤大小、部位和远处转移等均无关,P>0.05。IGF-1与IGFBP-3表达呈较弱的负相关性,r=-0.188,P=0.011。Logistic多因素回归分析显示,IGF-1在CRC中高表达是TNM分期增高和淋巴结转移的危险因素之一,IGFBP-3低表达是CRC发生淋巴结转移的危险因素之一。结论:IGF-1和IGFBP-3异常表达在CRC的发生发展过程中起重要作用,联合检测IGF-1和IGFBP-3可能成为临床上CRC评估预后新的生物学指标。
Objective: To investigate the expression of IGF-1 and IGFBP-3 in colorectal cancer (CRC) and its clinical significance. METHODS: A total of 183 CRC patients undergoing surgical treatment were selected as the observation group from the First Central Hospital of Baoding, the affiliated hospital of Chengde Medical College. Sixty-one patients were selected as normal control The expression of IGF-1 and IGFBP-3 in the two groups were detected by SP immunohistochemistry. Results: The positive rate of IGF-1 in CRC was 60.1% (110/183), significantly higher than that in control group (31.1%, 19/61), P <0.001. The positive rate of IGFBP-3 in CRC was 78.1% 143/183), significantly lower than 95.1% (58/61) in the control group, P = 0.003. The expression of IGF-1 was correlated with histological grade, depth of invasion, TNM stage and lymph node metastasis, P <0.05; IGFBP-3 expression was related to TNM staging and lymph node metastasis, P <0.05; both were related to age, sex, tumor size, And distant metastasis, etc. have nothing to do, P> 0.05. IGF-1 and IGFBP-3 expression showed a weak negative correlation, r = -0.188, P = 0.011. Logistic regression analysis showed that high expression of IGF-1 in CRC was one of the risk factors of TNM staging and lymph node metastasis. Low expression of IGFBP-3 was one of the risk factors of CRC in lymph node metastasis. Conclusions: Aberrant expression of IGF-1 and IGFBP-3 plays an important role in the development and progression of CRC. Combined detection of IGF-1 and IGFBP-3 may become a new biological indicator of clinical prognosis of CRC.