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目的了解山西省乙型肝炎病毒(HBV)的基因型及亚型分布情况。方法对136例乙型肝炎表面抗原(HBsAg)阳性者血清采用PCR-PFLP结合基因型特异性引物-PCR法进行HBV基因型及亚型检测。结果 136例HBV感染者的血清标本经PCR-PFLP分析,B基因型18例,均为Ba亚型,占13.2%;C基因型113例,占83.1%,除4例未分型外均为Ce亚型;D基因型5例,占3.7%。经型特异性引物-PCR分析,18例HBV B基因型中有4例为B/C基因型混合感染;5例经PCR-PFLP确定的D基因型病毒经型特异性引物法分析有2例扩增出E基因型条带。C、B基因型病毒感染者的平均年龄分别为(39.5±13.1)岁和(30.5±14.1)岁,差异有统计学意义(P<0.05);与B基因型相比,C基因型病毒感染者的HBeAg阴转率减慢。结论山西省HBV基因型主要为C(Ce),有少量的B(Ba)和D基因型,且存在B/C混合基因型,且可能存在D和E基因型病毒的重组体;与B基因型比较,感染C基因型病毒更难被机体清除,疾病更易慢性化。
Objective To understand the genotypes and subtypes of hepatitis B virus in Shanxi Province. Methods The genotypes and subtypes of HBV in serum of 136 patients with positive HBsAg were detected by PCR-PFLP and genotype-specific PCR-PCR. Results Serum samples from 136 HBV infected patients were analyzed by PCR-PFLP. Among the 18 B genotypes, all were B subtype, accounting for 13.2%; 113 C genotypes (83.1%), all except 4 Ce subtype; D genotype in 5 cases, accounting for 3.7%. According to the type-specific primer-PCR analysis, 4 of 18 HBV B genotypes were mixed with B / C genotypes. In 5 cases of D genotypes confirmed by PCR-PFLP, 2 were analyzed by type-specific primers E genotype amplified bands. The mean age of C and B genotypes was (39.5 ± 13.1) years and (30.5 ± 14.1) years, respectively, with significant difference (P <0.05). Compared with genotype B, genotype C virus infection The HBeAg negative rate slowed. Conclusion The genotypes of HBV in Shanxi province are mainly C (Ce), a small amount of B (Ba) and D genotypes exist, and B / C mixed genotypes exist, and recombinants of D and E genotype viruses may exist. In comparison, it is more difficult to be infected with genotype C virus by the body and the disease is more likely to be chronic.