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目的:观察胰岛素对大鼠肠缺血再灌注后小肠组织损伤的影响。方法:雄性SD大鼠40只随机分为4组,每组10只,手术对照组、单纯缺血组、再灌注组、胰岛素干预组。于30min缺血和120min再灌注后,进行组织病理学和生化检测。结果:(1)单纯缺血组肠粘膜损害较手术对照组明显升高(P<0.01),超氧化物歧化酶(SOD)活性无明显变化;(2)再灌注组SOD活性明显降低,与手术对照组和单纯缺血组相比较差异均有显著性(P<0.01);(3)胰岛素组SOD活性与再灌注组相比有明显改善(P<0.01)。结论:肠缺血可以引起肠粘膜损伤,再灌注则可加重这种损伤,胰岛素可以减轻再灌注损伤。
Objective: To observe the effect of insulin on intestinal tissue injury after intestinal ischemia-reperfusion in rats. Methods: Forty male Sprague-Dawley rats were randomly divided into 4 groups with 10 rats in each group. The rats in the control group, ischemia group, reperfusion group and insulin intervention group were randomly divided into four groups. After 30min ischemia and 120min reperfusion, histopathological and biochemical tests were performed. Results: (1) The damage of intestinal mucosa in ischemia group was significantly higher than that of operation control group (P <0.01), while the activity of superoxide dismutase (SOD) had no obvious change. (2) The activity of SOD in reperfusion group was significantly decreased There was significant difference between the operation control group and the ischemia group (P <0.01). (3) The activity of SOD in the insulin group was significantly improved compared with the reperfusion group (P <0.01). Conclusion: Intestinal ischemia can cause intestinal mucosal injury, reperfusion can increase this damage, insulin can reduce the reperfusion injury.