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研究秦岭辛家山林区红桦林细菌群落在土壤剖面上的分布状况,对评估土壤细菌在森林生态系统土壤肥力调节、碳氮循环等作用至关重要.采用Illumina MiSeq高通量测序技术对土壤细菌16S r DNA V3~V4可变区进行测序,结合相关生物信息学分析,初步探讨了红桦林0~10、10~20、20~40和40~60 cm这4个土壤层细菌群落丰富度、多样性指数和细菌群落组成及丰度变化.结果表明,在红桦林土壤剖面上,OTUs、Chao1指数、Shannon指数均在0~10 cm处达到最大值,分别为1 688、2 314、8.66,土层间差异不显著.4个土壤层的优势菌门均为酸杆菌门(Acidobacteria)、变形菌门(Proteobacteria),主要的优势菌属为Gp4、Gp6和Gp16.优势菌门的相对丰度在土层间并不相同,0~10 cm土壤层具有较高的变形菌门(Proteobacteria),其相对丰度为23.62%,而40~60 cm具有较高的酸杆菌门(Acidobacteria),相对丰度为62.88%.酸杆菌门(Acidobacteria)与全氮、土壤有机碳、C/N、可溶性有机碳显著相关,变形菌门(Proteobacteria)与土壤含水量、土壤有机碳、可溶性有机碳显著相关.经RDA分析证明,影响秦岭红桦林土壤剖面细菌群落分布的主要土壤因素是可溶性有机碳.这些研究结果表明在秦岭红桦林土壤4个土层均有较高的细菌多样性,为进一步认识森林土壤细菌多样性奠定了理论基础,在研究森林生态系统土壤剖面养分循环过程时应予以考虑.
It is very important to study the distribution of bacterial community in the soil profiles of the Betula platyphylla forest in Xinjiashan forest area of Qinling Mountains to evaluate the role of soil bacteria in regulating soil fertility and carbon and nitrogen cycling in forest ecosystems.Using Illumina MiSeq high throughput sequencing technology, Bacterial 16S r DNA V3 ~ V4 variable region was sequenced. Based on the bioinformatics analysis, the microbial community in four soil layers of 0 ~ 10, 10 ~ 20, 20 ~ 40 and 40 ~ 60 cm Degree, diversity index, bacterial community composition and abundance.The results showed that the OTUs, Chao1 index and Shannon index all reached the maximum at 0 ~ 10 cm in the profile of birch forest, respectively, which were 1 688, 2 314 , 8.66, no significant difference between soil layers.The dominant bacteria in four soil layers were Acidobacteria, Proteobacteria and the dominant dominant bacteria were Gp4, Gp6 and Gp16. The relative abundance was not the same among the soil layers. The 0-10 cm soil layer had higher Proteobacteria with a relative abundance of 23.62% and 40-60 cm with higher Acidobacteria ), Relative abundance was 62.88%. Acidobacteria and total nitrogen Soil organic carbon, C / N and soluble organic carbon were significantly correlated, and Proteobacteria was significantly correlated with soil water content, soil organic carbon and dissolved organic carbon. The RDA analysis proved that the distribution of bacterial community in the soil profile Of the main soil factors are soluble organic carbon.These results show that in the birch forest soil Qin Qinling have higher soil bacterial diversity in order to further understand the diversity of forest soil bacteria laid the theoretical foundation for the study of forest ecosystems Soil profile nutrient cycling should be considered.