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以高效液相色谱技术检测在北京和内蒙古种植的供试材料Harosoy近等基因系的低聚糖及其组分含量,考查Harosoy近等基因系的低聚糖含量的变异和不同地点材料的低聚糖及其组分含量相互间的相关性,低聚糖及其组分含量分别与蛋白质、脂肪含量间的相关性。研究结果表明,内蒙古种植材料低聚糖含量的平均值均高于北京种植的材料,说明内蒙古的条件有利于大豆的低聚糖形成和贮存。北京和内蒙古的材料蔗糖含量分布范围分别是3.3%~6.5%、3.9%~6.9%,棉籽糖分布范围分别是0.6%~1.4%、0.7%~1.1%,水苏糖分布范围分别是2.7%~3.7%、2.8%~3.8%,大豆低聚糖分布范围分别是6.9%~10.9%、7.8%~11.3%。同时发现,两地种植材料的低聚糖含量间和蔗糖含量间均具有显著负相关性,蔗糖含量间r=-0.7810,低聚糖含量间r=-0.7355;低聚糖及其组分含量与蛋白质、脂肪含量间均无相关性(P>0.05)。还发现了低聚糖及其组分含量在两地稳定表达的材料共10个。
The contents of oligosaccharides and their components in the near-isogenic lines of Harosoy, which were planted in Beijing and Inner Mongolia, were detected by HPLC. The variation of oligosaccharides content in Harosoy’s near isogenic lines and the low Glycan and its components, the correlation between the content of oligosaccharides and its components and protein, fat content. The results showed that the average content of oligosaccharides in planting materials in Inner Mongolia were higher than that in Beijing, indicating that the conditions in Inner Mongolia are favorable for the formation and storage of oligosaccharides in soybean. The range of sucrose content in Beijing and Inner Mongolia was 3.3% -6.5% and 3.9% -6.9% respectively. The distribution range of raffinose was 0.6% -1.4% and 0.7% -1.1%, respectively. The distribution range of stachyose was 2.7% ~ 3.7% and 2.8% ~ 3.8% respectively. The distribution range of soybean oligosaccharides were 6.9% -10.9% and 7.8% ~ 11.3% respectively. At the same time, it was found that there was a significant negative correlation between the content of oligosaccharides and the content of sucrose, the content of sucrose was -0.7810, the content of oligosaccharides was -0.7355, the content of oligosaccharides and its components And protein, fat content had no correlation (P> 0.05). Also found that oligosaccharides and its components in two stable expression of a total of 10 materials.