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目的探讨碲化镉量子点(CdTe QDs)对小鼠肾脏的氧化损伤作用。方法将40只雄性ICR小鼠随机分为5组,每组8只动物,尾静脉注射CdTe QDs溶液染毒。染毒组染毒浓度分别为0.5、5、50和500 nmol/ml,每只动物注射0.2 ml;对照组注射等体积的生理盐水,染毒24 h后小鼠脱臼处死。采用生化方法检测肾脏组织匀浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(MDA)含量,免疫组化法观察肾脏细胞8-羟基脱氧鸟嘌呤(8-OHdG)表达水平、TUNEL法检测肾细胞凋亡。结果 500 nmol/ml CdTe QDs染毒组MDA含量显著增加,与对照组比较差异有统计学意义(P<0.01);5、50和500 nmol/ml CdTe QDs染毒组SOD和CAT活性与对照组比较显著降低(P<0.01);免疫组化法观察0.5、5、50、500nmol/ml CdTe QDs染毒组肾细胞核内均可见棕褐色的8-OHd G阳性颗粒表达,TUNEL法检测可见50、500 nmol/ml CdTe QDs染毒组有凋亡细胞阳性颗粒表达。结论本实验条件下CdTe QDs可对小鼠肾脏组织产生一定程度的氧化损伤作用,并有一定的剂量-效应关系。
Objective To investigate the effects of cadmium telluride quantum dots (CdTe QDs) on the oxidative damage of kidney in mice. Methods Forty male ICR mice were randomly divided into five groups. Eight animals in each group were injected with CdTe QDs solution through tail vein. The exposure groups were exposed to 0.5, 5, 50 and 500 nmol / ml, 0.2 ml per animal respectively. The control group was injected with equal volume of normal saline and the mice were sacrificed 24 hours after exposure. The activities of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) in kidneys were detected by biochemical methods. The expression of 8-hydroxydeoxyguanine (8- OHdG) expression levels, TUNEL assay of renal cell apoptosis. Results Compared with control group, the content of MDA in CdTe QDs exposed group increased significantly (P <0.01). The activities of SOD and CAT in CdTe QDs exposed to 500 nmol / (P <0.01). The expression of 8-OHd G positive cells in the nuclei of all the cells exposed to 0.5, 5, 50, 500 and 500 nmol / ml CdTe QDs were detected by immunohistochemistry. The TUNEL assay showed 50, The apoptotic cells were positive in 500 nmol / ml CdTe QDs exposure group. Conclusion CdTe QDs can induce a certain degree of oxidative damage to the kidneys of mice and have a dose-response relationship.