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根据斑茅ITS区序列设计编号为EF1/ER1和编号为EF2 /ER2 的两对引物 ,经在甘蔗及其近缘属植物间ITS PCR扩增 ,证明这两对引物是斑茅特异引物 ,其中引物EF1/ER1在斑茅多态性研究中扩增出 5种带型 ,而引物EF2 /ER2 扩增出 3种带型 ;同时 ,利用这两对引物对甘蔗 (拔地拉 )与斑茅杂交的 9个F1,甘蔗斑茅真实杂种 (崖城 96 - 6 6、 96 - 46 )与CP84 - 1198的 36个BC1以及崖城 95 - 41与内江 5 7- 416的 12个BC1,15个曾被认为具有斑茅血缘的品系进行分子鉴定 ,结果表明 ,两对引物的鉴定结果基本一致 ,其中对斑茅F1的分子鉴定结果与前人 (沈万宽 )的同工酶鉴定结果相一致 ,对 15个曾被认为具有斑茅血缘的品系进行鉴定结果显示大部分品系为假杂种。
Two pairs of primers, EF1 / ER1 and EF2 / ER2, were designed according to the ITS region sequence of the locust. The two pairs of primers were identified as locus specific primers by ITS PCR amplification between sugarcane and its related genera Five EF1 / ER1 primers were amplified from the genomic DNA of the locus coeruleus, while three EF2 / ER2 primers were amplified from the EF2 / ER2 primer pairs. At the same time, Nine BC1 F1 hybrids of Saccharomyces cerevisiae, 12 BC1 and 15 BC1 clones of Acacia cuspidatum (Cliff 96-666, 96-46) and 36 BC1 of CP84-1989 and Yacheng 95-41 and Neijiang 5 7- 416 The results showed that the identification results of the two pairs of primers were basically the same, among which the molecular identification results of the dactylis F1 were consistent with those of the predecessor (Shen Wankuan) Fifteen lines that had been considered germline had been identified and most of the strains were fake hybrids.