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本研究从长期在空气中放置的沼液中分离得到1株可以耐受高浓度沼液的藻株,经形态和分子生物学方法鉴定为小球藻属的一种,命名为Chlorella sp.BWY-1.本研究所用的沼液来自于以固液分离后的猪场废水为发酵原料的沼气工程.与普通小球藻Chlorella regularis(FACHB-729)的对比研究表明,Chlorella sp.BWY-1在BG11和不同浓度的沼液中都有相对较强的生长速率、生物量积累能力和氮磷等污染物去除能力.Chlorella sp.BWY-1在BG11中有最高的生长速率和生物量生产力(324.40 mg·L-1,以dw计),但是其含油量和油脂生产力随着沼液浓度的增加而增加.在未稀释的沼液中Chlorella sp.BWY-1的含油量可达44.43%,油脂生产力达108.70 mg·L-1.分析结果表明该藻株在养殖废水处理和生物能源方面具有一定的应用潜力,可以结合固液分离、厌氧发酵等其他技术用于养殖场废水的处理和生物柴油的制取.
In this study, a strain of algae that can tolerate high concentrations of biogas slurry was isolated from long-term air-laid biogas slurry and identified as Chlorella sp. By the morphological and molecular biological methods. -1. The biogas slurry used in this study was from the biogas project of piggery wastewater after solid-liquid separation.Comparison with Chlorella regularis (FACHB-729) showed that Chlorella sp. WBY-1 In BG11 and different concentrations of biogas slurry, there was a relatively strong growth rate, biomass accumulation capacity and removal of pollutants such as nitrogen and phosphorus.Chlorella sp.BWY-1 had the highest growth rate and biomass productivity in BG11 ( 324.40 mg · L-1, dw), but its oil content and lipid productivity increased with the increase of biogas slurry concentration.The oil content of Chlorella sp. WBY-1 in undiluted biogas slurry reached 44.43% The results showed that the algae strain has potential application in aquaculture wastewater treatment and bioenergy, and can be used in the treatment of farm wastewater and other technologies combined with solid-liquid separation and anaerobic fermentation Preparation of biodiesel.